Home 5 Antiaging 5 Resistance Training Doubles NAD+ in Muscles of Middle-Aged, Overweight Subjects – Ten Week Study

Resistance Training Doubles NAD+ in Muscles of Middle-Aged, Overweight Subjects – Ten Week Study

Readers know that I promote Weight (or Resistance) Training as the most Anti-aging form of exercise known. Now comes a ten week study that shows a more than doubling of NAD+ in muscle tissue in middle-aged men and women, bringing them to levels equivalent to college-aged recreational athletes. Researchers in aging have considerable agreement that boosting NAD+ levels in older humans restores myriad physical functions and increases overall health. We can clearly use Nicontinamide Riboside (NR) or Nicotinamide Mononucleotide (NMN) to raise NAD+ levels and this has been shown to improve overall fitness. Now this can be coupled with resistance training to yield the benefits seen in this study, particularly increased strength and fitness.

“In the middle-aged participants, the 10-week training intervention: i) promoted training adaptations (i.e., increased strength and localized hypertrophy), ii) robustly increased muscle NAD+ and NADH concentrations, iii) modestly (but significantly) increased NAMPT protein levels and global SIRT activity, and iv) robustly increased citrate synthase activity levels in muscle suggesting mitochondrial biogenesis occurred.”

Biogenisis of mitochondria is really big deal; more mitochondria means more energy production in our cells (muscle cells in this case), but has a systemic impact, allowing virtually everything to function faster and better. With results like these, that we can get from a 1/2 hour in the gym a mere twice a week, why would we ever give up working out?

“This is the first evidence to suggest resistance training in middle-aged individuals restores muscle NAD+ and NADH concentrations to levels observed in recreationally-trained college-aged individuals. The finding of increased global SIRT activity is also novel, and the current data confirm that resistance training is capable of increasing muscle NAMPT protein levels. Finally, the training-induced increases in muscle mitochondrial density along with the association between increases in muscle citrate synthase activity and NAD+ levels are intriguing and warrant further investigation.”

You can read illuminating comments about this study and gain a better understanding of this study’s significance here at

You can also read the full study at

I jumped on NAD+ boosting, first with NR and then with NMN and have seen generalized benefits to every aspect of my health. Part of the proof of this can be seen in a video of my leg-press lifetime maximum, taken two weeks after my 80th birthday – check it out!   

To your greater health and fitness,


Frank Wilhelmi

Frank Wilhelmi

Frank Wilhelmi – Retired/consultant electronic engineer researches and reports practical strategies for optimizing health and fitness into advanced age. “I have a passion for living life to the fullest, and helping others to do the same.” A rapidly growing body of knowledge now enables us to extend our health and fitness decades beyond popular expectations.

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