Supplementing Soluble Klotho improves Markers of Aging in Mice
– And I’m reasonably sure it works in Humans as well!

• Vitamin D: Has vitamin D-responsive elements near the Klotho gene and has shown to upregulate Klotho expression in animal kidney cell lines and in humans with higher dietary intake. Note that some studies on actual supplementation in humans (e.g., dialysis patients) have shown mixed or even negative results, so the form and application may be critical.
• Vitamin C: A positive linear association between dietary vitamin C consumption and serum Klotho concentrations has been found in human population studies.
• Curcumin (from Turmeric): Demonstrated an ability to induce Klotho expression in human kidney cell lines in a lab setting.
• Resveratrol: Preclinical (rodent) studies found it significantly increased Klotho mRNA and protein levels in the kidney and brain.
• Ginseng: Increased Klotho expression in a mouse model of kidney injury.
• N-acetyl cysteine (NAC): Preserved Klotho expression in an animal model of induced kidney damage.
• Melatonin: Ameliorated Klotho deficiency effects in mice with kidney damage, partly due to its antioxidant properties.
• Folate (Vitamin B9): Increased dietary folate intake was significantly correlated with elevated serum Klotho in middle-aged and older adults in a population study. 

I was surprised to see no mention of Baicalin. Personally, I have used Baicalin, an extract from the herb Skullcap, available as a supplement in 500 mg/capsule, in doses up to 1000 mg/day for over 4 years to hopefully boost my plasma levels of s-klotho (soluble klotho). I get all of the items mentioned above in the Gemini response in Life Extension Mix, or sleep-related supplements, which I have taken daily for about 15 years.

I want to point out that I keep my measured 25-Hydroxy Vitamin D levels well above the suggested levels; typically in the range of 70-100 ng/ml, which I think has a significant benefit on klotho levels and immune function with aging. Klotho is primarily expressed in the kidneys and brain, and kidney’s production supplies most of the s-klotho for circulation in the blood to other parts of the body. There is plenty of evidence that higher blood levels improve aging in mammals, including humans, suggesting that an s-klotho supplement would be desirable.

To Your Greater Health and Fitness – and Longevity,

Frank